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Cells were transfected using lipofection (5 μ g DNA/mL, 5 μ L lipofectamine/mL) in OptiMEM medium for 4 h with either empty pIRES 2DsRed-Express2 bicistronic vector (Clontech, Mountain View, CA) or vector containing the sequence for amyloid-beta 1-42 (A β 1-42 )