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Cell-free supernatants were collected and stored at -20°C for the future assays and granulomas were harvested by adding ice-cold sterile 1x PBS to wells, followed by gentle scraping to achieve maximum recovery of granuloma lysates and stored at -20°C

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PubMed evidence trail Research sources used to frame this page For NAD+, MOTS-c, and 5-Amino-1MQ: separating hype from human data, FormBlends checks the page topic against primary trials, systematic reviews, guidelines, and current PubMed-indexed literature where available