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Mouse surface and intracellular antibodies Single-cell suspension was stained with anti-CD16/32 (BioXcell, 1 mg ml − 1 , 1:100) and conjugated antibodies against any combination of the following surface antigens: CD4 (RM4.5, 1:400), CD8 (53-6.7, 1:400), CD45 (30-F11, 1:400), TCRβ (H57-597, 1:200) and PD-1 (29F.1A12, 1:200)