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Common reconstitution volumes and resulting total peptide concentrations: 1mL BAC water: 80mg/mL total peptide (80,000 mcg/mL) — highly concentrated stock solution 2mL BAC water: 40mg/mL total peptide (40,000 mcg/mL) — standard research stock concentration 4mL BAC water: 20mg/mL total peptide (20,000 mcg/mL) — dilute stock for direct cell culture use at low volumes For most research applications, 2mL is the recommended starting volume, providing a concentrated stock that can be further diluted in cell culture media or assay buffer to target working concentrations in the nanomolar to micromolar range for individual components

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